This tutorial series describes how to design and manage a Breeding Management System (BMS) study planted with five doubled haploid (DH) maize lines. The scenario follows five promising DH maize lines newly introduced to a maize breeding program. The lines will be planted in a study of five non-randomized plots. The DH lines planted in the study will provide the basis for three activities.

1. Genotyping of the DH lines for assurance that the lines represent five unique genotypes and contain markers of interest.
2. Design a diallel crossing scheme for DH lines (including reciprocals and excluding selfs) to create hybrid seed. The hybrid seed is destined for a future study - a randomized performance trial.
3. Increase the DH seed by advancement.

Tutorial Steps

1. Reserve and withdraw DH seed from inventory.
2. Design study: 5 plots, single location, non-randomized
3. Design diallel crosses and generate hybrids in the database. Crosses are generative breeding methods expected to increase genetic diversity in offspring relative to the parents. 
4. Increase DH seed by advancing selfed ears to the next generation